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Lipids are a kind of bioorganic molecules that are difficult to dissolve in water but easy to dissolve in non-polar solvents. Lipids are the fundamental component of organisms, which is closely related to the activities of life. In recent years, more and more studied are focused on lipids, and the analysis of the structure and basic properties of lipids is the cornerstone of lipid research. Alfa Chemistry has accumulated a great deal of analytical experience by studying hundreds and thousands of lipids. Our laboratory equips with a variety of advanced analytical instruments, including mass spectrometry, liquid chromatography, gas chromatography, NMR, etc., to provide a comprehensive analysis service for different kinds of lipids. In addition, our analytical researchers have an in-depth understanding and research of lipids, and they will provide the most professional services to meet your any analytical needs.
Alfa Chemistry provides a comprehensive analysis service of lipids, and the details are as follows.
| Service items | Method summary |
| Physicochemical properties characterization of lipid (melting point, cloud point, flash point, density, content, rheology and etc.) | Each physical properties have corresponding measurement methods. For example, for cloud point test, a sample is heated to a temperature where all the crystals are known to have melted, then the sample is cooled at a controlled rate and the temperature at which the liquid just goes cloudy is determined. |
| Lipid composition determination | Various forms of chromatography are used to analyze the composition, e.g. thin layer chromatography (TLC), gas chromatography (GC), and high pressure liquid chromatography (HPLC). And the combined techniques such as GC-MS are also adopted, if necessary. |
| Structural characterization and identification of lipids | The proton NMR, carbon NMR, fourier transform infrared spectroscopy (FTIR), mass spectrometry (MS) and etc. are mainly used in the test. |
| Service items | Method summary |
| Phospholipids identification | The quantitative phosphorus NMR (QUANT 31P NMR) and qualitative phosphorus NMR (31P NMR) are used to identify the major phospholipid classes and their degradants in a sample and gives quantitative results. |
| Fatty acid characterization | Esterified fatty acids or total fatty acids are measured by gas chromatography. |
| Molecular characterization of unknown lipid samples | We will use our expertise and combine multiple methods for structural analysis of your unknown lipid samples. A common method is to determine the lipids of unknown samples using a combination of NMR and mass spectrometry. |
| Analytical method development, qualification and validation | We can design and develop a wide range of lipids assays method including NMR, HPLC-ELSD, LC-MS/MS and GC to ensure the accuracy of your analytical tests. |
