Pseudotargeted Lipidomics

Pseudotargeted lipidomics integrates the untargeted and targeted lipidomics strategies, i.e., using the targeted techniques based on the information from untargeted methods to achieve a high coverage lipidomics data acquisition. The strategy was proposed not long ago, and only a few studies have been reported on this technology. However, because it combines the advantages of untargeted lipidomics and targeted lipidomics, and shows the great application prospects. At present, studies on diseases (such as diabetes, depression) and food have used this technique and shown promising results. It is believed that pseudotargeted lipidomics will become an important tool in lipidomics research in the near future. Alfa Chemistry has a wealth of study experience in lipids, and our laboratory is equipped with the most advanced high resolution mass spectrometer, chromatographic separation instruments and professional analysis software. We will provide you with reliable, fast, cost-effective and one-stop pseudotargeted lipidomics analysis services. We look forward to cooperating with you!

Alfa Chemistry's Workflow

The complete analytical procedure of pseudotargeted lipidomics in Alfa Chemistry includes the following five steps ^[1]:

Step 1

First, according to different samples, the appropriate methods are adopted to carry out sample collection and pretreatment.

Step 2

Next, the untargeted lipidomics techniques are used to analyze mixed biological matrices of interest in full scan or IDA-based auto MS/MS scan to collect rich MS/MS fragment ions.

Step 3

After that, lipid ion pairs are defined based on the characteristic fragment ions and the corresponding parent ions generated from untargeted lipidomics analyses.

Step 4

And then, triple quadrupole-MS is used to realize high-coverage dynamic MRM analysis of the constructed lipid ion pairs.

Step 5

Finally, the data is extracted and then processed by software, and further analysis of its biological significance.

Advantages of Pseudotargeted Lipidomics

• The pseudotargeted lipidomics method shows satisfactory analytical characteristics in terms of linearity, precision, reproducibility, and recovery.
• The pseudotargeted lipidomics shows better repeatability and higher coverage of lipids than the untargeted lipidomics method.
• The pseudotargeted lipidomics achieves much more quantification of analytes than the targeted lipidomics profiling.

How to Order?

Reference

1. Xu T., et al. Recent advances in analytical strategies for mass spectrometry-based lipidomics[J]. Analytica Chimica Acta, 2020, 1137: 156-169.