Targeted Lipidomics

Targeted lipidomics focuses on characterization of specific lipids, often for quantification purposes. When you wish to study specific target lipids or lipid classes, targeted lipidomics is employed. In addition to, lipid classes with characteristic fragmentation patterns are suitable for targeted analysis, and low abundance lipids analysis also usually investigated via targeted analysis due to high sensitivity required ^[1]. Alfa Chemistry based on the sensitive and stable systems of separation, characterization, identification, analysis, and the professional technical teams to provide you with fast, accurate targeted lipidomics study service.

Alfa Chemistry's Analytical Strategies

The triple quadrupole (QQQ) mass spectrometer has been the standard workhorse in targeted lipidomics, as it offers good sensitivity, reproducibility, and a broad linear range. Based on this, Alfa Chemistry has built two analysis mode, namely, multiple reaction monitoring (MRM)—also known as selected reaction monitoring (SRM) and parallel-reaction monitoring (PRM), for targeted lipidomics service.

• MRM MS-based targeted lipidomics. In this mode, ionized lipids with specific mass-to-charge ratios (m/z) (precursor ions) are isolated at the first quadrupole (Q1). Thereafter, ions that produce specific fragment ions (product ions) are reselected at Q3 after fragmentation at Q2 by collision-induced dissociation (CID), which has been recognized as a gold standard for lipid quantification.
• PRM MS-based targeted lipidomics. In MRM the selected product ion, after fragmentation, is analyzed by a quadrupole (Q3), for PRM, Q3 is replaced by an Orbitrap. With this strategy, PRM not only has the MRM capability of selectivity by measuring a product ion, but also the qualitative capability because it measures the rest of the fragments. Moreover, the product ions are analyzed with high resolution and accuracy improving selectivity ^[2].

Alfa Chemistry's Workflow

The typical workflow of targeted lipidomics studies is as follows.

Service Scope

There are hundreds and thousands of lipids with wide diversities in structures and physiochemical properties in biological systems. Alfa Chemistry can offer the targeted lipidomics service for a lot of lipids, and they fall into the following eight categories.

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References

1. Lee H. C., and Yokomizo T. Applications of mass spectrometry-based targeted and non-targeted lipidomics[J]. Biochemical and Biophysical Research Communications, 2018, 504(3): 576-581.
2. Villasenor A.,et al. Analytical approaches for studying oxygenated lipids in the search of potential biomarkers by LC-MS[J]. TrAC Trends in Analytical Chemistry, 2021, 143: 116367.