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Asadi, Khatereh, et al. International Journal of Biological Macromolecules 265 (2024): 130654.
Trinitroglycerin (TNG) is a notable NO donor. Here, we synthesized TNG based on chitosan nanogels (Ngs) to mitigate complications related to high-dose TNG administration.
Preparation of Ngs and TNG-Ngs
Ngs were synthesized using an ion-gelation method by cross-linking chitosan (CS) with the polyanion tripolyphosphate (TPP). Specifically, at room temperature, CS (0.1%, w/v) was dispersed in an aqueous acetic acid solution (7 ml, pH = 4) under magnetic stirring (500 rpm) for 1 hour. The acetic acid concentration was 0.5% in the distilled water. Then, a TPP (0.07%, w/v) solution was added dropwise using a plastic Pasteur pipette. The reaction proceeded for 30 minutes, after which the suspension was centrifuged at 10,000×g for 10 minutes. The supernatant was discarded, and the precipitated Ngs were dispersed in distilled water.
For the preparation of TNG-Ngs, a 100 μ/ml TNG solution was added to the prepared chitosan solution, followed by the dropwise addition of the TPP solution. After centrifugation, the supernatant was collected for drug loading evaluation, and the precipitate was resuspended in distilled water.
